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基本信息

注意：

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載體描述

pQE-40 pQE vectors and constructs can be maintained in any E. coli strain that is ampicillin-sensitive and carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome. 配套表達(dá)菌株為M15[pREP4]感受態(tài)細(xì)胞。M15[pREP4]菌株攜帶有阻抑質(zhì)粒pREP4,該質(zhì)粒高水平的表達(dá)lac抑制子，在IPTG誘導(dǎo)前反式抑制蛋白表達(dá)。通過卡那霉素抗性篩選可以保持pREP4質(zhì)粒在菌株中的穩(wěn)定性。

DHFR是指二氫葉酸還原酶，CHO-DHFR細(xì)胞是二氫葉酸還原酶雙倍體基因缺失型CHO細(xì)胞，在缺乏DHFR的情況下無法生長，可以轉(zhuǎn)染表達(dá)DHFR的質(zhì)粒進(jìn)入該細(xì)胞系，這樣，轉(zhuǎn)染成功的細(xì)胞才能存活，其他細(xì)胞死亡，可以作為穩(wěn)定細(xì)胞系構(gòu)建的篩選系統(tǒng)。

載體序列

ORIGIN

        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca

       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga

      121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc

      181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc

      241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag

      301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta

      361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat

      421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt

      481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa

      541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt

      601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct

      661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttcc

      721 agatctgcat gcggtacccc gggtcgacct gcagccaagc ttaattagct gagcttggac

      781 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg

      841 gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag attttcagga

      901 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa

      961 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag

     1021 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt

     1081 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg

     1141 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc

     1201 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag

     1261 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct

     1321 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt

     1381 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa

     1441 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt

     1501 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg

     1561 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg

     1621 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg

     1681 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cctctagagc

     1741 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg

     1801 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg

     1861 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat

     1921 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg

     1981 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc

     2041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg

     2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag

     2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc

     2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag

     2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga

     2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc

     2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg

     2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt

     2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca

     2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca

     2641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag

     2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca

     2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg

     2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa

     2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta

     2941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag

     3001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga

     3061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac

     3121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc

     3181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta

     3241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac

     3301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat

     3361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa

     3421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg

     3481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag

     3541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc

     3601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct

     3661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat

     3721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg

     3781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc

     3841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta

     3901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg

     3961 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct

     4021 ttcgtcttca c

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